In Situ Hybridization (ISH) is a powerful technique that allows for specific localization of nucleic acid targets in fixed tissues, cells and circulating tumor cells (CTCs). The principle of ISH is that nucleic acids, when preserved adequately in a histologic specimen and when fixed appropriately, can be detected by using a nucleic acid probe, allowing you to obtain gene expression information in the context of tissue/cell morphology. In the past, quantitation of in situ gene expression, at the RNA (mRNAs or non-coding RNAs) level, had limited utility due to low sensitivity (e.g. non-radioactive formats), long complicated workflows (e.g. radioactive formats) and/or the inability to simultaneously multiplex.
ViewRNA Assays are possibly the most powerful and innovative tools you can bring to your pathology work. At Affymetrix, we are experts in designing assays that unlock the mysteries of the impact of coding and non-coding RNA on human development and disease. We can design and manufacture probes for any transcript you seek, including secreted proteins and non-coding sequences.Probes are manufactured to our quality consistency standards to ensure quantitative and reproducible results that you can trust time after time. Unique branched DNA detection technology delivers ultra-sensitive and highly specific signal. With ViewRNA Assays you can get the results you want.
We offer two basic ways to visualize your RNA targets in situ: chromogenic (CISH) and fluorescence (FISH) detection.
Chromogenic RNA in situ hybridization
Fluorescence RNA in situ hybridization
With ViewRNA Assays for RNA in situ hybridization you can enjoy
RNA in situ hybridization applications
Criteria for selecting the appropriate RNA in situ hybridization assay format:
*Part of the ViewRNA CTC Platform.**Full automation on Leica BOND RX - coming soon. Batch automation of the assay procedures can be achieved using the Little Dipper® Processor from SciGene