Fluorescence miRNA In Situ Hybridization (miRNA FISH) - Overview
Fluorescence based in situ hybridization (FISH) techniques are used to visualize DNA or localized RNAs within cells. However the fluorescence in situ hybridization of miRNA has always been limited by low sensitivity, complicated workflow and the inability to perform multiplex analysis. QuantiGene ViewRNA is a novel non-radioactive miRNA and mRNA in situ hybridization solution that offers single-molecule miRNA and RNA sensitivity. Affymetrix Research-Use-Only (RUO) products for miRNA FISH analysis allow investigators doing biomarker discovery or clinical research to validate and study miRNA biomarkers in suspension or adherent cells.
miRNA fluorescence in situ hybridization (FISH) assay – unique benefits
miRNA fluorescence in situ hybridization (FISH) assay – applications
QuantiGene ViewRNA miRNA In Situ Hybridization – Overview
miRNAs, small, non-coding molecules of 20-23 nucleotides, have emerged as key regulators in diverse biological processes that underlie the development and physiology of complex organisms. Recently, there is also increasing evidence implicating abnormal miRNA expression in human cancers and neurological disorders. Significant advances in the field of miRNA research have been made possible in part through the introduction of novel research tools, enabling investigators to discover, profile, quantify, validate, and functionally analyze miRNAs. However, the analysis of miRNAs in situ has been challenging and limited by sensitivity and specificity.
QuantiGene ViewRNA miRNA ISH Cell Assay overcomes these challenges by improving target retention and specificity by incorporating novel probe design with proprietary nucleic acid chemistry, and employing a second generation branched DNA (bDNA) signal amplification system to allow visual detection and quantitative validation of miRNA expression. The assay allows for simultaneous analysis of one miRNA and up to two mRNAs in the same assay, opening new avenues in miRNA research. Each punctated dot represents one molecule of the miRNA or mRNA enabling accurate quantitation of these targets.
Assay Performance Highlights
QuantiGene® ViewRNA miRNA ISH Cell Assay
|Sample Type||Adherent and suspension cells|
|Plex Level||1 miRNA and up to 2 mRNAs|
|Assay Format||24-well plates*|
|96-well optical bottom imaging plate|
|Instrumentation||Fluorescence microscope or imaging system|