QuantiGene^® ViewRNA In Situ Hybridization Overview

In Situ Hybridization (ISH) is a powerful technique that allows for specific localization of nucleic acid targets in fixed tissues, cells and circulating tumor cells (CTCs).  The principle of ISH is that nucleic acids, when preserved adequately in a histologic specimen and when fixed appropriately, can be detected by using a nucleic acid probe, allowing you to obtain gene expression information in the context of tissue/cell morphology. In the past, quantitation of in situ gene expression, at the RNA (mRNAs or non-coding RNAs) level, had limited utility due to low sensitivity (e.g. non-radioactive formats), long complicated workflows (e.g. radioactive formats) and/or the inability to simultaneously multiplex.

QuantiGene ViewRNA Assays are novel RNA in situ hybridization (ISH) assays enabling localization and visualization of RNA in cells or tissues in singleplex and multiplex assay formats. QuantiGene ViewRNA Assays offer sensitive single-molecule RNA detection with virtually no background.

We offer two basic ways to visualize your RNA targets in situ: chromogenic (CISH) and fluorescence (FISH) detection.

Chromogenic RNA in situ hybridization

Multiplex chromogenic in situ hybridization (CISH) enables you to visualize up to two RNA and lncRNA targets in the context of tissue morphology. RNA in situ hybridization assays for FFPE and frozen tissue sections QuantiGene ViewRNA ISH Tissue Assay - manual processing QuantiGene ViewRNA RX - automated processing on Leica BOND RX

Fluorescence RNA in situ hybridization

Multiplex fluorescence in situ hybridization (FISH) allows you to visualize up to four RNA and lncRNA targets in the context of tissue/cell morphology. You can analyze up to two RNA targets per tissue section and up to four RNA targets in cell samples. RNA in situ hybridization assays for FFPE and frozen tissue sections QuantiGene ViewRNA ISH Tissue Assay - manual processing QuantiGene ViewRNA RX - automated processing on Leica BOND RX RNA in situ hybridization assays for CTCs and cells QuantiGene ViewRNA CTC Platform QuantiGene ViewRNA miRNA ISH Cell Assay QuantiGene ViewRNA ISH Cell Assay - manual processing QuantiGene ViewRNA HC Screening Assay - automated processing

With QuantiGene ViewRNA Assays for RNA in situ hybridization you can enjoy

• Sensitive single-molecule detection of RNA
  
• High specificity using simultaneous branched DNA (bDNA) signal amplification and background suppression
  
• Flexible probe design to target any sequence within a gene
• “By request” probe synthesis to any target gene are delivered in less than 1 week
  

RNA in situ hybridization applications

• Transcriptional profiling of individual cells within a population
• Heterogeneity of gene expression in cells or tissues
• Stem cell pluripotency & differentiation
• Signaling pathways – cell to cell variability
• Quantifying gene expression in cancer biopsies
  
• A companion to antibody based in situ immunofluorescent and immunohistochemistry or DNA FISH assay

Criteria for selecting the appropriate RNA in situ hybridization assay format:

*Part of the QuantiGene ViewRNA CTC Platform.