In Situ Hybridization (ISH) is a powerful technique that allows for specific localization of nucleic acid targets in fixed tissues, cells and circulating tumor cells (CTCs). The principle of ISH is that nucleic acids, when preserved adequately in a histologic specimen and when fixed appropriately, can be detected by using a nucleic acid probe, allowing you to obtain gene expression information in the context of tissue/cell morphology. In the past, quantitation of in situ gene expression, at the RNA (mRNAs or non-coding RNAs) level, had limited utility due to low sensitivity (e.g. non-radioactive formats), long complicated workflows (e.g. radioactive formats) and/or the inability to simultaneously multiplex.
QuantiGene ViewRNA Assays are novel RNA in situ hybridization (ISH) assays enabling localization and visualization of RNA in cells or tissues in singleplex and multiplex assay formats. QuantiGene ViewRNA Assays offer sensitive single-molecule RNA detection with virtually no background.
We offer two basic ways to visualize your RNA targets in situ: chromogenic (CISH) and fluorescence (FISH) detection.
Chromogenic RNA in situ hybridization
Fluorescence RNA in situ hybridization
With QuantiGene ViewRNA Assays for RNA in situ hybridization you can enjoy
RNA in situ hybridization applications
Criteria for selecting the appropriate RNA in situ hybridization assay format:
*Part of the QuantiGene ViewRNA CTC Platform.**Full automation on Leica BOND RX - coming soon. Batch automation of the assay procedures can be achieved using the Little Dipper® Processor from SciGene