Affymetrix | Panomics Solutions
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Quantitative Biology Delivered.

Unique Benefits

  • The QuantiGene Plex Assay provides DIRECT from lysate measurement of 3 to 80 target RNAs per well with unparalleled accuracy and precision.
  • Quantitatively measure multiple RNA targets simultaneously with unparalleled accuracy and precision

  • Quantitation of original RNA population directly from lysates avoids biases, sample loss, false positive/negative results associated with
    • RNA isolation
    • cDNA synthesis
    • PCR amplification
  • High accuracy and precision using branch DNA (bDNA) signal amplification versus target amplification (PCR)
    • High precision - coefficient of variation (CV) of less than 15% for entire process (sample isolation to results)
    • High accuracy - distinguish percentage differences in RNA copy number - linear assay with low CVs
  • No optimization required for multiplexing 3 to 80 target RNAs

  • Excellent results in difficult clinical research samples, including, H&E-stained FFPE, blood, and skin

Accurate results in a variety of samples

  • Compatible with human, animal and plant tissues, FFPE tissue sections, whole blood, Tempus blood, PAXgene blood, cultured cells, isolated RNA and more

Validated panels

  • Each panel is functionally validated

Applications

  • Validation and testing of disease biomarkers (e.g. cancer, neurology, autoimmune disease, etc.)
  • Validation of microarray or sequencing results
  • Predictive toxicology
  • Secondary screening
  • Pathway analysis

QuantiGene Plex Assay Specifications

  Limit of Detection   ≤ 1,000-2,000 transcripts/assay well
  Limit of Quantitation   ≤ 2,000-4,000 transcripts/assay well
  Linear Dynamic Range   ≥ 3.0 logs
  Assay CV   ≤ 15% intra-assay;≤ 20% inter-assay
  Compatible Sample Types   Cultured cells, bacteria, whole blood,
 PAXgene blood or dried blood spots,
 fresh/frozen tissues (animal or plant),
 FFPE samples, purified RNA
  Assay Format   96-well plate
  Targets/well   3 - 80