Press Release

New array for mapping the network of transcription factor interactions.

Redwood City, Calif. September 9, 2003—Panomics, Inc. introduces the TF Protein Array. This new array represents a significant improvement over traditional methods like pull-down assays, co-immunoprecipitation, super-gel shift, and the yeast two-hybrid system. A single hybridization experiment reveals how a protein of interest interacts with 54 transcription factor proteins immobilized on an array membrane.

To create the TF Protein Array, 54 transcription factor proteins were expressed from full-length cDNAs and immobilized on an array membrane. In a simple hybridization with the array, a protein of interest can be used as bait to search for interactions with the immobilized proteins. Interactions can be assessed either with an antibody against the protein of interest, or by tagging the protein. The signal is visualized via chemiluminescent detection.

The TF Protein Array is an ideal tool for mapping the intricate network of interactions between proteins. When used in conjunction with the TF-TF Interaction Arrays, this array facilitates identification of nearest-neighbor interactions between transcription factors, as well as distant interactions.