Procarta^® TF Plex Assays

• Panomics Offers one 40 Plex Panels and a second 43 plex panel for a total of 83 Luminex assays
• Profile the DNA binding activity of up to 43 different transcription factors per Panel (TFs) in a single well
• Mix and Match to create your own plex set from 3-42 Plex
• Quantitate TF binding activity from whole cell lysates or nuclear extracts
• Complete assay in 5 hours

Transcription factors (TFs), which play crucial roles in the regulation of gene expression in the human genome, are highly regulated by a variety of mechanisms. A single extracellular stimulus can trigger multiple signaling pathways, and these in turn can activate multiple TFs to mediate the inducible expression of target genes. Alterations in the activities of TFs are often associated with human diseases, such as altered activating factor 1, estrogen receptor, and p53 function in cancer, nuclear factor kB in inflammatory diseases, and peroxisome proliferator-activated receptor gamma in obesity. A systematic assay for profiling the activation of TFs will aid in elucidating the mechanisms of TF activation, reveal altered TFs associated with human diseases, and aid in developing assays for drug discovery. We have developed a 40-plex fluorescent microsphere-based TF activation assay system with a 96-well plate format. The assay system enables high-throughput profiling of the DNA binding activity of TFs in multiple samples with high sensitivity.

Procarta Transcription Factor assays combine two technologies: Panomics' patent-pending detection probe separation method and xMAP® (multi-analyte profiling beads). Together, these two technologies enable the quantitative measurement of DNA binding activity of up to 40 transcription factors (human, mouse or rat) from as little as 500 ng of protein from a whole cell lysate or 250 ng of protein from a nuclear extract. The xMAP system, developed by Luminex Corp., combines flow cytometry, fluorescent-dyed microspheres (beads), lasers and digital signal processing to effectively allow multiplexing of up to 100 unique assays within a single sample.

Monitoring PMA-Mediated Induction in Nuclear Extracts

Panomics Procarta Transcription Factor Assay was used to simultaneously measure the activity of 40 TFs in a single assay well. HeLa cells were serum starved overnight and subsequently stimulated PMA or a vehicle control for 4 hours. As expected, PMA induced AP1 and NF-E2 and the activity of NF-1 and STAT4 were unchanged. Sample inductions from the Procarta TF assay were confirmed by EMSA Gel Shift assays. Lane 1 = Free Probe; Lane 2 = HeLa Nuclear Extract; and Lane 3 = PMA Induced HeLa Nuclear Extract.

TF Plex Panel 1

Panomics offers 40 different Transcription factors for Panel 1. Users can choose from the 40 TF's below to create their own Plex set.

TF Plex Panel 2

Panomics offers 43 different Transcription factors for Panel 1. Users can choose from the 43 TF's below to create their own Plex set.

Brochures:

• Tools for Transcription Factor Research Brochure (PDF)
• Luminex Assays From Panomics (PDF)
• Procarta Transcription Factor Assay Presented at 2007 Luminex xMAP Meeting (PDF)

Manuals:

• Procarta TF Assay Kit User Manual (PDF)
• Procarta TF Nuclear Extraction Kit User Manual (PDF)
• Procarta TF Whole Cell Lysate Kit User Manual (PDF)

Frequently Asked Questions:

• Procarta TF Assay Kit Frequently Asked Questions (PDF)

Product Inserts:

• Procarta TF Assay Kit Product Insert 40-plex Panel 1 (PDF)
• Procarta TF Assay Kit Product Insert 3-39 Plex Panel 1 (PDF)
• Procarta TF Assay Kit Product Insert 43-plex Panel 2 (PDF)
• Procarta TF Assay Kit Product Insert 3-42 Plex Panel 2 (PDF)
• Procarta TF Sample Diluent Kit Product Insert (PDF)
• Procarta TF Control Nuclear Extract Kit Product Insert (PDF)
• Procarta TF Control Whole Cell Lysate Kit Product Insert (PDF)

Related Products:

• TF ELISA Kits
• Protein/DNA Arrays
• EMSA Gel Shift Assays
• Stable Cell Lines

Publications

Yaoi et al 2006 Development of a microsphere multiplexed high throughput assay (PDF)