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Panomics’ AP1 Reporter Stable Cell Line is a robust tool for high-throughput analysis of in vivo drug efficacy and specificity. The cells also provide a reproducible, ready-to-use method of evaluating uncharacterized growth factors, extracellular stimuli and assessment of signaling pathway convergence upon AP1.
AP1 background
The activator protein 1 (AP-1) transcription factor is a dimeric complex that comprises members of the JUN, FOS, ATF and MAF protein families with FOS and JUN being the most common proteins in mammalian cells. AP-1 activity is induced by growth factors, cytokines and oncoproteins. Upon activation, AP-1 binds to the TPA Responsive element (TRE) and induces transcription of a variety of genes involved in multiple cellular functions such as proliferation, survival, differentiation and transformation.
Regulation of AP-1 is complex and occurs at different levels that include the composition of the dimers, the expression level of each monomer and post-transcriptional modification of the protein. In addition the AP-1 proteins interact with ancillary proteins that also regulate the activity of the complex. AP1 can be phosphorylated at the N-terminal region of the c-Jun protein by Jun N-terminal kinase (JNK). Activation of JNK is mediated by activation of a signalling pathway including the small GTPase Rac and protein kinases MEK kinase (MEKK) and JNKK. In addition, AP1 can be activated by the induction of c-JUN transcription. c-JUN is a cellular immediate-early gene whose transcription is increased rapidly in response to external stimuli such as growth factors. This increase does not require new protein synthesis. c-JUN is rapidly induced in cultured cells in response to certain stimuli such as epidermal growth factor (EGF), serum, 12-O-tetradecanoyl phorbol-13-acetate (TPA), nerve growth factor, and UV.
With Panomics’ AP1 Reporter Stable Cell Line you can accurately monitor any changes occurring along the AP1 pathway. The stable cell line is derived from human 293T cells with chromosomal integration of a luciferase reporter construct regulated by 3 copies of the AP1 response element. This clonal cell line is obtained by cotransfection of pAP1-luc and pTK-hyg followed by hygromycin selection at 200 ug/ml. The cells have been tested with PMA for induction of luciferase activity.
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